Production of therapeutic proteins through plant tissue and cell culture
REZA S. GHARELO, EBRAHIM D. OLIAEI, ALI BANDEHAGH, EHSANEH KHODADADI, POUYA M. NOPARVAR
Nowadays, pharmaceutical recombinant protein is increasingly used in treatment of many diseases such as hepatitis, anemia, diabetes and cancer. Different protein expression systems have been used for the expression of recombinant proteins in which each of them face obstacles that make utilizing them as comprehensive expression system in order to express wide variety of proteins difficult.
Plant cell as a eukaryotic expression system have many advantages compared to other hosts. They are very "safe" and significantly decrease concerns about the contamination of recombinant proteins with human pathogens. In addition to this, plants as eukaryotic expression system perform proper post-translational modification, in case of eukaryotic proteins, and appropriate folding resulting in right function in biological environments. Therefore, the production of pharmaceutical protein through plant cells can be absolutely promising approach.
In this review, the production of pharmaceutical protein in plant cells, advantages and disadvantages, offered methods and techniques for developing recombinant protein yields, and affective factors on the whole process of pharmaceutical protein expression in the molecular level will be reviewed.
Key words: bioreactor, plant suspension culture, recombinant protein